How to grow bacteria cultures in a petri dish. Petri dish and its history Petri dish play with cheats for weight
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Probably, in order to gain the trust and affection of users of different age categories, an online browser game must be perfect, maybe that's why Agario is just that. Otherwise, there is simply no explanation for such an unusually high popularity. Perhaps Agar.io gathers the public just because it is free and without registration. You need to spend only a few seconds to start playing, and if you have already started, then in the future it is simply impossible to come off.
So what exactly is this app?
You are a molecule in a petri dish. You need to eat other bacteria that are smaller than you, and after gaining the maximum amount of weight, you get to the top and become a leader.
And finally, about cheats, they simply don't exist. The administration makes sure that no programs appear to hack this game. That is why it acquires uniqueness and peculiarity.
But not everything is so hopeless, there are some cheats that help to quickly achieve the cherished goal.
Thanks to them, the process of the origin and development of life on Earth will appear before you in the form of a fascinating visual online miniature. Believe me, the Petri Dish game is a wonderful opportunity to observe how a cell develops, how it grows, interacts with the outside world, what difficulties it faces. Moreover, you yourself can take part in this exciting action by starting to play with a virtual Petri dish.
The essence of natural selection
Since we are talking about evolution in this series of games, your ward will be the simplest form of life - a cage. She is quite active, but so far not great, and therefore defenseless. Over time, the cell, of course, will grow and get stronger, but for now it needs protection, and you need care and ingenuity in managing it. The latter quality is a must for the success of this series of games. From the point of view of gameplay, playing Petri Dish is very, very simple: just move your ward with the cursor across the screen - and no problem.
Primitive graphics only reinforce this illusion. In fact, a rather complex multi-step strategy awaits you, in which you cannot do without actively using your brains. And do not forget that in this game everything is like in nature: the strong eats the weak, due to this it develops and grows. He has no other sources of resources. Therefore, while you are weak, you need to be very careful not to swim close to large cells. And if you blunted and nevertheless approached them, try to quickly implement the salvation plan, thought out in advance to the smallest detail. How, there is no plan? Then the "monsters" will devour you and will not notice. Improvisation in this case will only make it worse.
By the way, you can save yourself in two ways: by hiding behind green ball cells dotted with thorns (their presence in the game is completely controlled by the developers, they do not control the users of these greenish things) and by forced dividing your cell into two halves (for this, quickly press the space bar). In the first case, the hungry participants in the game simply will not get you, in the second, they will only eat half (the second will be able to escape at this time).
Well, in order to grow up yourself, you also need to actively "eat" your smaller brothers. You can start with plankton (clusters of tiny dots on the screen), and then move on to cells controlled by other players.
You can play Petri Dish for as long as you want if you follow two simple rules: there are those who are smaller than you, and do not forget that with an increase in size, the speed of any cell decreases. And if your new impressive parameters seem attractive to some starving giant cage, you will hardly be able to escape from it. But you can try to split using a space into as many parts as you want. In general, the Petri Dish game is an endless field for experiments and testing the effectiveness of different tactics. She will grab all your attention for a long time and will not let you get bored.
Pure crops
In 1872, Robert Koch, the future great microbiologist, was appointed sanitary doctor in Wolstein (now Wolsztyn in Poland), where anthrax was rampant at that time. This disease has been known since ancient times under the name "sacred fire": people believed that only angry gods could send such punishment to earth. Anthrax was a threat to all agriculture - livestock most often fell ill. However, not only animals died, but also people: farmers, shepherds, milkmaids.
Examining the blood of dead animals through a microscope, Koch discovered that only one microbe is to blame for the development of the disease - the bacillus ( Bacillus anthracis). The scientist managed to isolate the bacillus and grow pure culture- a culture represented by one type of microbes. He infected a perfectly healthy animal with pure culture, causing him anthrax. The scientist realized that the creation of pure cultures is the key to successfully determining the causes of infection.
Robert Koch, like his predecessors, grew bacteria in a liquid medium - meat or cereal broths. Koch managed to get a pure culture of the anthrax bacillus in a liquid broth, but he looked for another method. There were good reasons for this. If several types of bacteria got into the broth, they mixed with each other and it was extremely difficult to separate them. I had to transplant bacteria more than once. Koch took a small droplet from the solution, where the necessary bacteria were the most, and transferred it to fresh broth. In the new broth, there were already fewer foreign bacteria, but this operation had to be monotonously repeated several times, so that as a result, only one type of microbes appeared in the nutrient medium.
Robert Koch(1843-1910) - German microbiologist. He discovered anthrax bacillus, Vibrio cholerae and tuberculosis bacillus (Koch's bacillus). In 1905 he was awarded the Nobel Prize in Physiology or Medicine "for research and discoveries concerning the treatment of tuberculosis."
Before Robert Koch, researchers observed microorganisms as colorless, which led to numerous errors. Koch used aniline dyes that selectively stain only microbes. After Koch's experiments, scientists around the world began to invent methods for staining bacteria. So, in 1884, the doctor Hans Christian Gram came up with a staining method, which became one of the main ones for determining the presence and type of bacteria in a certain substrate.
Why are pure cultures needed?
Microbes are very small (on average 0.5–5 µm), do not differ much in appearance, which causes certain difficulties in their study. For research, you need to isolate a microbe from the surrounding world, full of a variety of microorganisms. A microbial cell, once on a nutrient medium, gives rise to offspring - a clot of identical cells, a colony that can be studied as one microorganism. It turned out that by choosing the cultivation conditions, cells of any microorganism can be obtained in pure form. This means giving it a name, describing its properties, classifying it. Thanks to this discovery by Robert Koch, microbiology was singled out as an independent science.
Solid environment
After reporting on the discovery of the anthrax pathogen, Robert Koch was invited to head a laboratory at the Microbiological Institute in Berlin and was offered the position of adviser to the Ministry of Health. Koch now has good equipment, talented assistants and the ability to solve a riddle that has long tormented him. It was known that tuberculosis is also caused by some kind of microbe: it was possible to infect healthy animals with the tissues of a sick person. Koch managed to find a technique for staining tissue preparations in order to see the pathogen bacterium through a microscope. But his joy was short-lived - the bacterium did not want to grow on conventional nutrient media.
Macro view of tubercle bacillus colonies ( Mycobacterium tuberculosis). They are distinguished by a colorless uneven surface.
Once a scientist noticed a moldy potato thrown on the table with an abundance of multi-colored specks-colonies - gray, yellow, green. He collected samples from each colony and saw through a microscope that each speck is a colony of one kind of microbes! In a liquid medium, microbes mixed and it was extremely difficult to separate them. And on a solid environment they stayed in one place, multiplied and gave a pure culture!
Koch's accidental observation made a revolution: fresh potatoes became one of the first solid media for growing microorganisms. However, this nutrient medium is not suitable for all microbes, so the search for an alternative solid medium continued.
Koch again zealously began to cultivate a tuberculosis culture. The bacteria did not grow on the potato slices. Then he began to use gelatin to turn the broth into a solid nutrient medium. After many unsuccessful attempts, Koch added blood serum to the medium to recreate the conditions of a living organism. After 15 days (unprecedentedly long for the anthrax bacillus), droplets of colonies of the dangerous tuberculosis bacillus appeared on the surface of the medium.
Microorganisms divide every 20 minutes, therefore, already 3 hours after transplanting microbes on a Petri dish, you can see colonies, and after a day the number of bacteria in them is in the millions.
Microbiological art
Alexander Fleming invented a new art form - painting with bacteria on a solid medium. Fleming, a member of the Chelsea Art Club, invented amateur watercolors. The scientist painted graceful ballerinas, luxurious houses, and soldiers with bacteria. The creation of the picture required accuracy: it is necessary not only to find bacteria with different pigments, but also to choose the growing time so that monochromatic ones grow at the same time and do not violate the color boundaries.
The American Society for Microbiology (ASM), inspired by the example of Fleming and his colleagues, has been hosting the Agar Art Competition since 2015. Talented microbiologists and artists paint real pictures. Some come up with their own plots, others reproduce paintings by masters, for example, "Starry Night" by Van Gogh.
In 1928, British bacteriologist Alexander Fleming discovered that a colony of molds had grown on agar in one of the Petri dishes next to the bacteria. The bacterial colonies around the molds turned pale - their cells were destroyed. Fleming isolated a substance from molds that destroys bacterial cells - penicillin, the first antibiotic. Fleming's discovery changed not only medical science, but the fate of many seemingly hopeless patients.
Talented laboratory assistant
At that time, the only laboratory flask that was suitable for growing microbes was a test tube. But handling it required skill: if you put the test tube horizontally, the uncured medium will spill, put it at an angle, it can fall and break. The chance of contracting a dangerous disease multiplied before our eyes! Then Koch and his laboratory assistants came up with the idea of pouring the culture medium into cups and covering them with tall glass jars. But to look at the colonies, the cap had to be removed, and this is a direct route of infection. It was then that the finest hour of one of the laboratory assistants came - Julius Petri... He reduced the height of the walls of the cup in which the microbes were grown, leaving the low sides. And instead of a huge cap, he covered it with another transparent cup - this way it turned out to be more convenient to observe the colonies.
Petrie worked under Koch's leadership for only a couple of years (1877–1879), but during this time he radically influenced the future of microbiology. Julius Petri's innovation gave the strongest impetus to medicine and saved millions of lives. After working under the leadership of Robert Koch, he headed the Gobersdorf sanatorium - the first European center for the treatment of tuberculosis patients.
Agar agar
In Koch's laboratory, not only a Petri dish appeared, but also a standard filling for it - agar nutrient medium. Its predecessor, a medium containing gelatin, melted easily when heated, like jellied meat placed in a warm place. Colonies on such an environment turned into mush.
Agar-based medium invented Walter Hesse, another Koch laboratory assistant. He entered the service with his wife Fanny. She was not listed as a laboratory employee, but did the work of a scientific illustrator - she sketched microorganisms that she saw through a microscope.
Fanny made a jelly one day. Hesse noticed that it does not melt in the sun and retains its shape. He found out that the main component of jelly is agar-agar - a substance that is extracted from red and brown algae. Walter replaced it with standard gelatin, and the bacterial culture media became harder. Agar-agar is still used for the preparation of media today, but it is purified in a special way.
To determine the effectiveness of antibiotics in the pharmaceutical industry, special tests are used. For example, the agar diffusion method. Microorganisms are inoculated into Petri dishes. Discs containing certain doses of different antibiotics are placed on the seeded surface at an equal distance from each other. The larger the radius of the growth inhibition zone, the more effective the drug is against this microorganism.
At first, the Petri dish was used only for growing cell cultures, but now this dish is used in various fields of science. For example, the effectiveness of antibiotics is studied on Petri dishes, they are used to study food safety and to grow genetically engineered bacteria that synthesize the insulin patients with diabetes need.
Petri dishes are made in different sizes and from a wide variety of materials - glass, plastic and even stainless steel. For work, a scientist can choose the right one.
In order to start gaining mass, you need to devour other players on one side and befriend a few of them on the other. Meetings and even parties can be arranged in the Petri Dish. You can team up with several players, work out a common strategy and make your way to the top of the food chain.
Someone is constantly present in the game, gatherings are arranged, streamers appear, various competitions from the administration, and so on. Skins are maximally tailored for clients, and their choice is simply huge. There is no cheating, hidden fees or bots in the game. All cheating players are instantly recorded and blocked by the administration.
The game begins with the fact that we choose the map on which your hero will be born, and the mode in which you are ready to play. The more people on the map, the more difficult it is to play, the less - the easier. As for the modes, these are HardCore, MegaSplit, FFA and others. After you have chosen a mode, read its rules (they are located on the right). Different cards also differ in the limits and amount of food, so you also need to deal with the card from the very beginning.
There are a lot of buttons involved in the Petri Dish, although in order to understand what needs to be pressed first and what you can wait with, you need to understand which processes are paramount and which are less important. One of the most important processes is the division of the player into parts in the event of a collision with a large and dangerous enemy. Space is responsible for the separation. Feeding and shooting are controlled by the same button - W. Sometimes the game is paused (button F), when the danger of being eaten by another player is too great. The V button, for example, is responsible for firing a virus. The compass is turned on and off with the E key, the full screen is turned on with F11, and you can exit the menu with the Escape key.
Purpose of the game
The goal of the game is quite simple - to teach any gamer to work, both in a team and individually. Interaction is actively practiced in the game, both between players and between the latter and the administration.
In addition, the game trains your reaction and decision-making speed. If you have not been able to think globally and build a line of survival and advancement in the game until now, now you will gradually master it! All processes practiced by the game are very relevant for real life too!
